KIYAKUBE 3D Cell Culture Accessories

Click on a topic to learn more about 3DKUBE™ 3D Cell Culture Accessories.

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Universal Microscope Stage Adapter (BUY NOW!)
Model AC010

Adaptable to all conventional microscope stages
Same dimensions as a standard microscope slide
Secures 3DKUBE™ 3D Cell Culture Plasticware
for in situ 3D laser confocal microscopy
Useful as a stand during fluid perfusion experiments

Spectrometer Stage Adapter (BUY NOW!)
Model AC020

Conveniently aligns 3DKUBE™ 3D Cell Culture Plasticware into a conventional 6-well plate configuration
Useful for conducting in situ colorimetric, fluorometric and luminescent analysis
Useful as a multi-sample holder for prep and transport

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Male Luer Plugs (BUY NOW!)
Model FC010

Gamma irradiated
Provides convenient and aseptic plugging of inlet/outlet perfusion ports for transport or storage
4 plugs per pack
6 packs per unit (24 plugs total)

Syringe Pump Connector Kit (BUY NOW!)
Model FC030

Gamma irradiated
Pre-assembled tubing and luer connectors
Provides convenient assembly of a closed flow circuit from syringe-to-3DKUBE-to-medium reservoir

Three-way Stopcock Valve (BUY NOW!)
Model FC050

Gamma irradiated
When included in a flow circuit, the 3-way valve provides a convenient sampling port to aliquot cell culture medium for offline analysis

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The following diagrams demonstrate perfusion flow circuits used with 3DKUBE™ 3D Cell Culture Plasticware (circled in blue). A variety of pumping mechanisms (i.e., peristaltic, syringe, gravity) can be used to perfuse the 3D cell-scaffold construct contained within the culture chambers. Common luer connectors, stopcock valves and tubing can be used to customize the perfusion flow circuit to accommodate the desired experimental protocol.
One-way Perfusion with Syringe Pump & Collection Reservoirs	Advanced Perfusion Flow Circuit with Peristaltic Pump & Medium Reservoirs, 3-way Valves, and Syringe Pump & Collection Reservoirs (Only 1 of 2 flow circuits shown)
Reversible Perfusion with Peristaltic Pump & Medium Reservoirs

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(Click thumbnail for larger image)

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● General User Guide	*● In Situ* Histology Embedding User Guide**
*● In Situ* Imaging User Guide**	● Perfusion Flow Circuit Common Parts List
*● In Situ* Biochemical Assays User Guide**	● Frequently Asked Questions (FAQ)
● White Paper - "Novel Plasticware for 3D Cell Co-culture"
*● White Paper - "3D Cell Culture in situ* Histological Embedding"**
*● White Paper - "Cell Viability in 3D Culture using in situ* Bioluminescence"**